Measures spike latencies in response to pulses close to the firing threshold.

Author

Jan Benda

Version

1.2 (Nov 03, 2010)

Options

• Test-Pulse
• durationsel=in milliseconds: Set duration of stimulus (string)
• duration=100ms: Duration of stimulus (number)
• durationfac=1tau_m: Duration of stimulus (number)
• startamplitudesrc=custom: Set initial amplitude to (string)
• startamplitude=0.1nA: Initial amplitude of current stimulus (number)
• startamplitudestep=0.1nA: Initial size of amplitude steps used for searching threshold (number)
• amplitudestep=0.01nA: Final size of amplitude steps used for oscillating around threshold (number)
• adjust=DC: Adjust (string)
• First Pre-Pulse
• preduration=0ms: Duration of first pre-pulse stimulus (number)
• preamplitudesrc=custom: Set amplitude of first pre-pulse to (string)
• preamplitude=0.1nA: Amplitude of first pre-pulse stimulus (number)
• prevcamplitude=0mV: Voltage clamp value of first pre-pulse (number)
• prevcgain=0mS: Gain for voltage clamp of first pre-pulse (number)
• prepulseramp=none: Start first pre-pulse with a ramp (string)
• prepulserampwidth=0ms: Width of the ramp (number)
• Second Pre-Pulse
• pre2duration=0ms: Duration of second pre-pulse stimulus (number)
• pre2amplitudesrc=custom: Set amplitude of second pre-pulse to (string)
• pre2amplitude=0.1nA: Amplitude of second pre-pulse stimulus (number)
• Post-Pulse
• postduration=0ms: Duration of post-pulse stimulus (number)
• postamplitudesrc=custom: Set post-pulse amplitude to (string)
• postamplitude=0.1nA: Amplitude of post-pulse stimulus (number)
• Timing
• searchpause=500ms: Duration of pause between outputs during search (number)
• pause=1000ms: Duration of pause between outputs (number)
• delay=50ms: Time before stimullus onset (number)
• savetracetime=500ms: Length of trace to be saved and analyzed (from test-pulse on) (number)
• repeats=10: Repetitions of stimulus (integer)

Key shortcuts

• M: Start measuring now.

Possible stimulus configurations are shown in the figure:

The left column shows the stimuli without a DC input whereas in the right column stimuli starting out from a preset DC input (for example by the RePro SetDC) are shown. Stimuli A and B are the standard test protocol, whereas for testing out the dynamics stimuli F and G might be most usefull.